diff --git a/packages/angular/build/src/builders/karma/application_builder.ts b/packages/angular/build/src/builders/karma/application_builder.ts
index 40f66a9c6773..b2f4e4bba95c 100644
--- a/packages/angular/build/src/builders/karma/application_builder.ts
+++ b/packages/angular/build/src/builders/karma/application_builder.ts
@@ -84,7 +84,7 @@ class AngularAssetsMiddleware {
       const file = this.latestBuildFiles.files[pathname];
 
       if (file?.origin === 'disk') {
-        this.serveFile(file.inputPath, undefined, res);
+        this.serveFile(file.inputPath, undefined, res, undefined, undefined, /* doNotCache */ true);
 
         return;
       } else if (file?.origin === 'memory') {
diff --git a/packages/angular/build/src/builders/karma/tests/behavior/rebuilds_spec.ts b/packages/angular/build/src/builders/karma/tests/behavior/rebuilds_spec.ts
index e740b7adfcd6..6ec02c2c28f1 100644
--- a/packages/angular/build/src/builders/karma/tests/behavior/rebuilds_spec.ts
+++ b/packages/angular/build/src/builders/karma/tests/behavior/rebuilds_spec.ts
@@ -10,6 +10,7 @@ import { concatMap, count, debounceTime, distinctUntilChanged, take, timeout } f
 import { execute } from '../../index';
 import { BASE_OPTIONS, KARMA_BUILDER_INFO, describeKarmaBuilder } from '../setup';
 import { BuilderOutput } from '@angular-devkit/architect';
+import { randomBytes } from 'node:crypto';
 
 describeKarmaBuilder(execute, KARMA_BUILDER_INFO, (harness, setupTarget) => {
   describe('Behavior: "Rebuilds"', () => {
@@ -68,5 +69,59 @@ describeKarmaBuilder(execute, KARMA_BUILDER_INFO, (harness, setupTarget) => {
 
       expect(buildCount).toBe(expectedSequence.length);
     });
+
+    it('correctly serves binary assets on rebuilds', async () => {
+      await harness.writeFiles({
+        './src/random.bin': randomBytes(1024),
+        './src/app/app.component.spec.ts': `
+            describe('AppComponent', () => {
+              it('should fetch binary file with correct size', async () => {
+                const resp = await fetch('/random.bin');
+                const data = await resp.arrayBuffer();
+                expect(data.byteLength).toBe(1024);
+              });
+            });`,
+      });
+
+      harness.useTarget('test', {
+        ...BASE_OPTIONS,
+        watch: true,
+        assets: ['src/random.bin'],
+      });
+
+      interface OutputCheck {
+        (result: BuilderOutput | undefined): Promise<void>;
+      }
+
+      const expectedSequence: OutputCheck[] = [
+        async (result) => {
+          // Karma run should succeed.
+          expect(result?.success).withContext('Initial test run should succeed').toBeTrue();
+          // Modify test file to trigger a rebuild
+          await harness.appendToFile(
+            'src/app/app.component.spec.ts',
+            `\n;console.log('modified');`,
+          );
+        },
+        async (result) => {
+          expect(result?.success).withContext('Test should succeed again').toBeTrue();
+        },
+      ];
+
+      const buildCount = await harness
+        .execute({ outputLogsOnFailure: true })
+        .pipe(
+          timeout(60000),
+          debounceTime(500),
+          concatMap(async ({ result }, index) => {
+            await expectedSequence[index](result);
+          }),
+          take(expectedSequence.length),
+          count(),
+        )
+        .toPromise();
+
+      expect(buildCount).toBe(expectedSequence.length);
+    });
   });
 });